The sequencing and characterization of chloroplast genome of Geranium sibiricum Linne

Abstract

The complete chloroplast genome of Geranium sibiricum Linne. was sequenced, assembled and annotated. It is a circular form of 150,656 bp in length, which was separated into four distinct regions, a large single copy (LSC) of 73,862 bp, a small single copy region (SSC) of 52,666 bp, two inverted repeats (IR) of 12,064bp. A total of 124 genes were predicted, of which, 87 encode proteins, 4 rRNA, 33 tRNA. The evolutionary history was inferred using Maximum Likelihood method, and the result indicates that G. sibiricum was grouped within Geraniaceae, and comprised a clade with Geranium palmatum under 100% Bootstrap value.

Keywords

• geranium sibiricum
• chloroplast genome
• phylogenetic analysis