Nature Communications (Jan 2023)
High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform
- Cheng-Yong Tham,
- LaiFong Poon,
- TingDong Yan,
- Javier Yu Peng Koh,
- Muhammad Khairul Ramlee,
- Vania Swee Imm Teoh,
- Suihan Zhang,
- Yi Cai,
- Zebin Hong,
- Gina S. Lee,
- Jin Liu,
- Hai Wei Song,
- William Ying Khee Hwang,
- Bin Tean Teh,
- Patrick Tan,
- Lifeng Xu,
- Angela S. Koh,
- Motomi Osato,
- Shang Li
Affiliations
- Cheng-Yong Tham
- Cancer Science Institute of Singapore, National University of Singapore
- LaiFong Poon
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- TingDong Yan
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- Javier Yu Peng Koh
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- Muhammad Khairul Ramlee
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- Vania Swee Imm Teoh
- Cancer Science Institute of Singapore, National University of Singapore
- Suihan Zhang
- Department of Microbiology and Molecular Genetics, University of California
- Yi Cai
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- Zebin Hong
- Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, (A*STAR)
- Gina S. Lee
- National Heart Centre Singapore, Duke-NUS Medical School
- Jin Liu
- Centre for Quantitative Medicine, Duke-NUS Medical School
- Hai Wei Song
- Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, (A*STAR)
- William Ying Khee Hwang
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- Bin Tean Teh
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- Patrick Tan
- Cancer Science Institute of Singapore, National University of Singapore
- Lifeng Xu
- Department of Microbiology and Molecular Genetics, University of California
- Angela S. Koh
- National Heart Centre Singapore, Duke-NUS Medical School
- Motomi Osato
- Cancer Science Institute of Singapore, National University of Singapore
- Shang Li
- Cancer and Stem Cell Biology Program, Duke-NUS Medical School
- DOI
- https://doi.org/10.1038/s41467-023-35823-7
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 13
Abstract
There exist challenges in quantifying the length of individual telomeres at single nucleotide resolution. Here the authors report a method to capture telomeres and accurately analyse their length in human cell lines and patient peripheral blood leukocyte samples using single-molecule real-time sequencing.
