International Journal of Nanomedicine (Oct 2021)

Bombesin Peptide Conjugated Water-Soluble Chitosan Gallate—A New Nanopharmaceutical Architecture for the Rapid One-Pot Synthesis of Prostate Tumor Targeted Gold Nanoparticles

  • Tangthong T,
  • Piroonpan T,
  • Thipe VC,
  • Khoobchandani M,
  • Katti K,
  • Katti KV,
  • Pasanphan W

Journal volume & issue
Vol. Volume 16
pp. 6957 – 6981

Abstract

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Theeranan Tangthong,1,2 Thananchai Piroonpan,2 Velaphi C Thipe,3,4 Menka Khoobchandani,4,5 Kavita Katti,4,5 Kattesh V Katti,4– 6 Wanvimol Pasanphan1,2 1Department of Materials Science, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand; 2Center of Radiation Processing for Polymer Modification and Nanotechnology (CRPN), Department of Materials Science, Faculty of Science, Kasetsart University Chatuchak, Bangkok, 10900, Thailand; 3Department of Chemistry, University of Missouri, Columbia, MO, 65211, USA; 4Institute of Green Nanotechnology, University of Missouri, Columbia, MO, 65211, USA; 5Department of Radiology, University of Missouri, Columbia, MO, 65211, USA; 6Department of Physics, University of Missouri, Columbia, MO, 65211, USACorrespondence: Wanvimol PasanphanDepartment of Materials Science, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan Road, Lat Yao, Chatuchak, Bangkok, 10900, ThailandTel +66 2562 5555 (Ext. 646518)Email [email protected] V KattiInstitute of Green Nanotechnology, University of Missouri, Columbia, MO, 65211, USATel +1 573 882-5656Fax +1 573 884-5679Email [email protected]: We report herein bombesin peptide conjugated water-soluble chitosan gallate as a template for rapid one-pot synthesis of gold nanoparticles (AuNPs) with capabilities to target receptors on prostate cancer cells.Methods: Water-soluble chitosan (WCS), anchored with gallic acid (GA) and LyslLys3 (1,4,7,10-tetraazacyclo dodecane-1,4,7,10-tetraacetic acid) bombesin 1– 14 (DBBN) peptide, provides a tumor targeting nanomedicine agent. WCS nanoplatforms provide attractive strategies with built-in capabilities to reduce gold (III) to gold nanoparticles with stabilizing and tumor-targeting capabilities. WCS-GA-DBBN encapsulation around gold nanoparticles affords optimum in vitro stability.Results: The DBBN content in the WCS-GA-DBBN sample was ∼ 27%w/w. The antioxidant activities of WCS-GA and WCS-GA-DBBN nanocolloids were enhanced by 12 times as compared to the nascent WCS. AuNPs with a desirable hydrodynamic diameter range of 40– 60 nm have been efficiently synthesized using WCS-GA and WCS-GA-DBBN platforms. The AuNPs were stable over 4 days after preparation and ∼ 3 days after subjecting to all relevant biological fluids. The AuNPs capped with WCS-GA-DBBN peptide exhibited superior cellular internalization into prostate tumor (PC-3) cells with evidence of receptor mediated endocytosis.Conclusion: The AuNPs capped with WCS-GA-DBBN exhibited selective affinity toward prostate cancer cells. AuNPs conjugated with WCS-GA-DBBN serve as a new generation of theranostic agents for treating various neoplastic diseases, thus opening-up new applications in oncology.Keywords: water-soluble chitosan, gallic acid, bombesin, one-pot synthesis, gold nanoparticle, prostate cancer

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