F1000Research (Sep 2024)

The identification of high-performing antibodies for FUS (Uniprot ID: P35637)  for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry [version 3; peer review: 2 approved]

  • Peter S. McPherson,
  • Carl Laflamme,
  • Kathleen Southern,
  • Riham Ayoubi,
  • Maryam Fotouhi,
  • Walaa Alshalfie,
  • Harvinder Virk,
  • Zhipeng You,
  • Carolyn Jones,
  • Michael Biddle

Journal volume & issue
Vol. 12

Abstract

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RNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations in the C-terminal domain region, where the nuclear localization signal (NLS) is located, causes the redistribution of FUS from the nucleus to the cytoplasm. In neurons, neurotoxic aggregates are formed as a result, contributing to neurogenerative diseases. Well-characterized anti-FUS antibodies would enable the reproducibility of FUS research, thereby benefiting the scientific community. In this study, we characterized ten FUS commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

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