SPERMOVA (Aug 2020)

Determination of the freezing of hair rams (Pelibuey-Blackbelly) according to sperm viability and kinetics

  • Julio Cesar Gómez Vargas,
  • Rosendo Cuicas Huerta,
  • Isidro Jáuregui Plata,
  • Isidro Gutiérrez Segura,
  • Raúl Ulloa Arvizu,
  • David Urban Duarte,
  • Efrén Estrada Paqui

DOI
https://doi.org/10.18548/aspe/0008.04
Journal volume & issue
Vol. 10, no. 1
pp. 26 – 31

Abstract

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The objective of this study was to categorize the freezing of sperm of 11 rams (PelibueyBlackbelly) aged 18 to 24 months based on total motility and sperm viability at thawing. The semen was diluted to 37 °C with Triladyl® and egg yolk, packed in 0.5 mL straws at a sperm concentration of 200 million. After 15 minutes after defrosting at 37 °C, sperm motility and kinetics were evaluated by means of the CASA system, and fluorescence viability with kit FluoVit® (Microptic, S.L., Barcelona, Spain). The freezing categorization of ram ejaculates was 27.2% and 72.7% for rams with high freezing (CAC) (n= 3) and rams with low freezing (CBC) (n= 8) respectively. Ejaculates from CAC showed significant differences (p<0.05) in kinetic speed parameters (VCL, VSL and VAP), which suggests that these ejaculates are more suitable for cryopreservation and would have a greater chance of fertilizing due to their faster and more progressive movements.

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