The Study of Inhibition Effects Satureja khuzestaniea Essence against Gene Expression bap Acinetobacter baumannii with Real time PCR Technique

Abstract

Background and Aim: Acinetobacter baumannii a major hospital pathogens and causes outbreaks of infections and associated to nosocomial infections, including bacteremia, pneumonia, meningitis, urinary tract infection, and wound infections. Satureja khuzestaniea province the group Nnayyan and Carvacrol and thymol are antimicrobial activity of this plant. Biofilm-related genes (bap)-specific proteins on the cell surface generate a direct role in biofilm formation and infection of the bacteria is eliminated. The aim of this study was to investigate the effect of alcoholic extract Satureja khuzestaniea in gene expression (bap) was A. baumanii if it can be as effective as a complementary treatment or therapy in infections caused by these bacteria and inhibit virulence genes used. Materials and Methods: In this study the effect of Satureja khuzestanica essence, an endemic plant of Iran, on the expression level of bap gene in A. baumannii were investigated. For this purpose, MIC was determined for A. baumanii . Then, bacteria were treated with S. khuzistanica essence. The bap genes expression in treated and non-treated bacteria, before and after treatment was evaluated using Real time PCR technique. Results: Surprisingly, the expression level of bap gene was decreased in the presence of S. khuzestanica. However, the expression of DNA gyrA gene that was used as an internal control was not altered before and after treatment with this herb. Conclusions: Based on the results, S. khuzestanica could play a, major role in lowering the A. baumannii resistance to drugs, by reducing bap gene expression. According to results of current research we hope in future be used it to the clinic with a wider range as a complementary therapy and also for surgery operation.

Keywords

• Acinetobacter baumannii
• bap gene
• Real time PCR