Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging

S-C. A. Yeh; J. Hou; J. W. Wu; S. Yu; Y. Zhang; K. D. Belfield; F. D. Camargo; C. P. Lin

doi:10.1038/s41467-022-27973-x

Nature Communications (Jan 2022)

Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging

S-C. A. Yeh,
J. Hou,
J. W. Wu,
S. Yu,
Y. Zhang,
K. D. Belfield,
F. D. Camargo,
C. P. Lin

Affiliations

S-C. A. Yeh: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School
J. Hou: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School
J. W. Wu: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School
S. Yu: Department of Chemistry and Environmental Science, New Jersey Institute of Technology
Y. Zhang: Department of Chemistry and Environmental Science, New Jersey Institute of Technology
K. D. Belfield: Department of Chemistry and Environmental Science, New Jersey Institute of Technology
F. D. Camargo: Stem Cell Program, Boston Children’s Hospital
C. P. Lin: Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School

DOI: https://doi.org/10.1038/s41467-022-27973-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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The fate of hematopoietic stem cells can be controlled by factors such as calcium ion concentration. Here the authors report an intravital ratiometric analysis method to measure extracellular calcium ion concentrations and absolute pH in mouse bone marrow.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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