Response of Glycine max to Azospirillum lipoferum y Rhizobium etli at 50% dose of nitrogen fertilizer

Abstract

The production of Glycine max (soybean) demands nitrogenous fertilizer (NIFE), as a NH4NO3 (ammonium nitrate) to supply its N demand, which when is applied in excess causes lost soil`s productivity. An alternative for this problem is to reduce and to optimize NIFE, with inoculants based in genus and species of plant growth promoting bacteria (PGPB). The aim of this research was to analyze G. max response to Azospirillum lipoferum and Rhizobium etli at 50% dose of NIFE. In that sense experimental was conducting by 5 treatments with NIFE at 100% and 50% doses for G. max inoculated with PGPB, response was based on its phenology: plant height (PH) and root length (RL) and its biomass: shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW), root dry weight (RDW). Experimental data were analyzed by ANOVA and Tukey HSD p<0.05%. Results indicated a positive respond of G. max with A. lipoferum and R. etli at seedling level was registered 0.90 g of SDW, this numerical value was statistically different than 0.16 g of SDW of G. max not inoculating, fed with mineral solution with 100% of NIFE dose, used as relative control (RC). At level of flowering G. max responded to A. lipoferum and R. etli registered 3.17 g of RFW this numerical value was statistically different compared to 1.79 g of RFW of G. max or RC. This data suggests that the positive respond of G. max was due to both PGPB able to transform radical exudates in phytohormones to improve radical absorption of N and optimize NIFE reduced at 50% dose, showing health growing and at the same preventing soil`s lost productivity

Keywords

• soil
• nife
• glycine max
• azospirillum lipoferum
• rhizobium etli
• root exudates
• phytohormones.