Effects of L-serine on oxidative stress indices and histopathological changes of pancreas in streptozotocin-induced diabetic mice

Abstract

Background and Aim: Diabetes mellitus is a metabolic disorder with an increasing global prevalence that can lead to premature death. High blood glucose is the main lab. abnormality in diabetes mellitus which occurs as a consequence of disorder in pancreatic insulin secretion or function. L -Serine supplementation regarded as safe by the FDA can improve glucose homeostasis and reduce homeostasis model assessment-estimated insulin resistance (HOMA-IR) and oxidative stress. The aim of this study was to investigate the effects of L-serine intake on oxidative stress indices and histopathologic changes in the pancreas of diabetic mice. Materials and Methods: In order to conduct this study, 18 c57bl/6 male mice were purchased and divided into 3 groups (control, diabetic control and diabetic mice treated by L-serine(. Diabetes induced by chemical method (streptozotocin, 200 mg/kg). After four weeks of oral administration of L-Serine (approximately 280 mg/day/mouse), animals were euthanized by guillotine and blood samples and pancreas tissues were obtained to determine biochemical parameters, oxidative stress indices and pathological changes. Results: The results of this study showed that oral administration of the supplement of L-Serine in diabetic mice could help to lower blood sugar levels and could lead to increased catalase enzyme activity (P<0.05) but had no significant effect on the levels of MDA, cholesterol, and triglyceride. On the other hand, histopathological changes showed a slight reduction in diabetes-induced pancreas damage in mice treated with L-serine. Conclusions: These findings showed that the supplement of L-Serine may have a protective effect against diabetes-induced pancreas damage by lowering blood sugar and improving oxidative stress status.

Keywords

• diabetes
• l-serine
• pancreas
• oxidative stress
• histopathology
• amino acid