Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing

Sheng-Nan Chang; Jien-Jiun Chen; Jo-Hsuan Wu; Yao-Te Chung; Jin-Wun Chen; Chu-Hsuan Chiu; Chia-Ju Liu; Meng-Tsun Liu; Yi-Cheng Chang; Chin Li; Jou-Wei Lin; Juey-Jen Hwang; Wen-Pin Lien

doi:10.3390/cells11010098

Cells (Dec 2021)

Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing

Sheng-Nan Chang,
Jien-Jiun Chen,
Jo-Hsuan Wu,
Yao-Te Chung,
Jin-Wun Chen,
Chu-Hsuan Chiu,
Chia-Ju Liu,
Meng-Tsun Liu,
Yi-Cheng Chang,
Chin Li,
Jou-Wei Lin,
Juey-Jen Hwang,
Wen-Pin Lien

Affiliations

Sheng-Nan Chang: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Jien-Jiun Chen: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Jo-Hsuan Wu: Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California, San Diego, CA 92093, USA
Yao-Te Chung: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Jin-Wun Chen: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Chu-Hsuan Chiu: Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan
Chia-Ju Liu: Department of Nuclear Medicine, National Taiwan University Hospital, Taipei 100, Taiwan
Meng-Tsun Liu: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Yi-Cheng Chang: Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan
Chin Li: Department of Biomedical Sciences, National Chung-Cheng University, Chia-Yi 62102, Taiwan
Jou-Wei Lin: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Juey-Jen Hwang: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan
Wen-Pin Lien: Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan

DOI: https://doi.org/10.3390/cells11010098
Journal volume & issue: Vol. 11, no. 1
p. 98

Abstract

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Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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