Possible role of pomegranate fruit in reversing renal damage in rats exposed to Phenylhydrazine

Abstract

Background: Pomegranate granatum (molasses and peels) and its constituents showed protective effects against natural toxins such as phenylhydrazine as well as chemical toxicants as arsenic, diazinon, and carbon tetrachloride. Aim: The current study was aimed to assess the effect of pomegranate molasses, white peel extract; red peel extract on nephrotoxicity induced by Phenylhydrazine. Methods: 80 male rats were divided to eight equal groups; a control group, pomegranate molasses pure group, White peel pomegranate pure group, red peel pomegranate pure group, Phenylhydrazine (PHZ) group, pomegranate molasses + Phenylhydrazine group, White peel pomegranate + Phenylhydrazine group and red peel pomegranate + Phenylhydrazine group. Kidney function, inflammation markers, antioxidant activities and renal tissues histopathology were investigated. Results: The results revealed that Phenylhydrazine group showed a significant increase in LDH, MDA, creatinine, uric Acid, blood urea nitrogen, C - reactive protein (CRP), Tumor Necrosis Factor, thiobarbituric acid reactive substances, and total antioxidant capacity with a significant decrease of CAT, GPx, and SOD as compared with Control group. Other pomegranate treated and PHZ co-treated groups with pomegranate showed significant decreasing of LDH, MDA, creatinine, uric Acid, blood urea nitrogen, tumor necrosis factor, thiobarbituric acid reactive substances and total antioxidant capacity with a significant increase of CAT, GPx, and SOD as compared with Phenylhydrazine group. Conclusion: Collectively our data suggest that red, white peels and molasses have anti-toxic and anti- inflammatory effect on renal function and tissues. [Open Vet J 2023; 13(10.000): 1268-1276]

Keywords

• peel pomegranate
• molasses
• phenylhydrazine
• thiobarbituric acid reactive substances
• total antioxidant capacity