Effect of Iodoform in Maize and Clover Grass Silages: An In Vitro Study

Abstract

Iodoform has been shown to be an enteric methane-reducing agent. This study aimed to investigate if iodoform differentially affected maize (MS) and clover grass silage (GS). These substrates were used in four 22 h in vitro gas production fermentations with and without 0.032 mg/g of dry matter substrate of 0.064% iodoform in zeolite (IZ). The results of organic matter degradation (dOM, g/g), volatile fatty acids (VFAs, mMol/L), partitioning factor (PF, mg/mL), total gas production (TGP, mL/g OM), and periodic hydrogen and methane yield (mL/g OM) were evaluated. The interaction between substrate and IZ for dOM was significant with significantly less in GS. The interaction between substrate and additive was continuously significant for TGP. The additive in GS numerically decreased the TGP, but significantly increased TGP in MS. The interaction between substrate and additive was sporadically significant with methane, increasing the yield when IZ was added to MS, but a consistent, non-significant, decrease in yield was found when IZ was added to GS. Total VFA production and propionate content increased in both substrates with the addition of IZ, but only significantly in GS. Iodoform significantly decreased the efficiency of microbial conversion (PF) in both substates. Differential effects of iodoform on GS and MS were evident. More research is needed to determine the modes of action of iodoform.

Keywords

• degradation
• fermentation
• in vitro gas production
• iodoform
• methane