Frontiers in Bioengineering and Biotechnology (Jul 2020)

Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes

  • Rui-Rui Guo,
  • Gerard Comamala,
  • Huan-Huan Yang,
  • Marius Gramlich,
  • Ya-Min Du,
  • Ting Wang,
  • Anne Zeck,
  • Kasper Dyrberg Rand,
  • Li Liu,
  • Josef Voglmeir

DOI
https://doi.org/10.3389/fbioe.2020.00741
Journal volume & issue
Vol. 8

Abstract

Read online

Peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins. So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties. Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance. One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases. The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.

Keywords