Transcriptome analysis of sea cucumber (Apostichopus japonicus) in southern China under heat stress

Qiuhua Yang; Yanfang Zhong; Fuyuan Yang; Huiyao Li; Ngoc Tuan Tran; Ming Zhang; Lili Wang; Libin He; Zhe Zhang; Hui Ge; Jianshao Wu; Chunxiang Ai; Shengkang Li; Qi Lin

Aquaculture Reports (Jun 2024)

Transcriptome analysis of sea cucumber (Apostichopus japonicus) in southern China under heat stress

Qiuhua Yang,
Yanfang Zhong,
Fuyuan Yang,
Huiyao Li,
Ngoc Tuan Tran,
Ming Zhang,
Lili Wang,
Libin He,
Zhe Zhang,
Hui Ge,
Jianshao Wu,
Chunxiang Ai,
Shengkang Li,
Qi Lin

Affiliations

Qiuhua Yang: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China; Corresponding authors at: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China.
Yanfang Zhong: Institute of Marine Sciences, Shantou University, Shantou 515063, China
Fuyuan Yang: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Huiyao Li: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China
Ngoc Tuan Tran: Institute of Marine Sciences, Shantou University, Shantou 515063, China
Ming Zhang: Institute of Marine Sciences, Shantou University, Shantou 515063, China
Lili Wang: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China
Libin He: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China
Zhe Zhang: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China
Hui Ge: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China
Jianshao Wu: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China
Chunxiang Ai: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China
Shengkang Li: Institute of Marine Sciences, Shantou University, Shantou 515063, China
Qi Lin: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China; Fisheries Research Institute of Fujian, Xiamen 361013, China; Fisheries College, Jimei University, Xiamen 361021, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; Corresponding authors at: Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China.

Journal volume & issue: Vol. 36
p. 102036

Abstract

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Sea cucumber (Apostichopus japonicus) is a significant aquatic economic species globally. However, the advancement of the sea cucumber aquaculture industry is impeded by its high susceptibility to elevated temperatures. To elucidate the impact of high temperature on physiological changes, gene expression, and metabolic responses in sea cucumbers, we conducted transcriptional profiling of A. japonicus under heat stress. Our results revealed 3691 differentially expressed genes (DEGs), comprising 1629 up-regulated and 2062 down-regulated genes, between the control and treatment groups. Analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Protein-Protein Interaction Networks (PPI) indicated that high-temperature stress triggered the expression of stress defense-related genes while inhibiting the expression of genes associated with development and metabolism. Furthermore, 30 out of 39 DEGs were annotated to heat shock protein genes, underscoring the crucial role of these genes in the regulatory mechanism of A. japonicus in response to heat stress. In summary, our findings provide fundamental insights for further investigation into the molecular mechanisms in A. japonicus under high-temperature stress and may contribute to the development of novel strategies for identifying heat-tolerant candidate genes through the analysis of their transcriptional changes in the future.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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