MILT DILUTION EFFECTIVENESS ON PIKEPERCH (SANDER LUCIOPERCA) SPERM DNA INACTIVATION

Abstract

Percid fishes, including pikeperch (Sander lucioperca) have recently become the subject of intense research. In order to obtain gynogenetic all female pikeperch populations, normal pikeperch eggs are fertilized with inactivated sperm. Because pikeperch semen has a high viscosity, milt has to be diluted in an immobilizing solution before DNA inactivation. The aim of this study was to assess milt diluting solutions effectiveness in order to inactivate sperm DNA with UV irradiation, to produce meiotic gynogenetic pikeperch (Sander lucioperca). We assessed sperm motility after dilution in 5 different immobilizing solutions. Best results were obtained using Ringer’s solution as pikeperch sperm diluent.

Keywords

• pikeperch
• milt
• dilution
• dna inactivation