Transgenic Anopheles mosquitoes expressing human PAI-1 impair malaria transmission

Tales V. Pascini; Yeong Je Jeong; Wei Huang; Zarna R. Pala; Juliana M. Sá; Michael B. Wells; Christopher Kizito; Brendan Sweeney; Thiago L. Alves e Silva; Deborah J. Andrew; Marcelo Jacobs-­Lorena; Joel Vega-Rodríguez

doi:10.1038/s41467-022-30606-y

Nature Communications (May 2022)

Transgenic Anopheles mosquitoes expressing human PAI-1 impair malaria transmission

Tales V. Pascini,
Yeong Je Jeong,
Wei Huang,
Zarna R. Pala,
Juliana M. Sá,
Michael B. Wells,
Christopher Kizito,
Brendan Sweeney,
Thiago L. Alves e Silva,
Deborah J. Andrew,
Marcelo Jacobs-Lorena,
Joel Vega-Rodríguez

Affiliations

Tales V. Pascini: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Yeong Je Jeong: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Wei Huang: Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health
Zarna R. Pala: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Juliana M. Sá: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Michael B. Wells: Department of Cell Biology, Johns Hopkins University School of Medicine
Christopher Kizito: Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health
Brendan Sweeney: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Thiago L. Alves e Silva: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Deborah J. Andrew: Department of Cell Biology, Johns Hopkins University School of Medicine
Marcelo Jacobs-Lorena: Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health
Joel Vega-Rodríguez: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

DOI: https://doi.org/10.1038/s41467-022-30606-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

Read online

Plasmodium gametes and sporozoites activate surface-bound plasminogen to plasmin that degrades extracellular matrix barriers, therewith facilitating parasite motility in mosquitoes and mammalian hosts. To control malaria transmission, Pascini et al. generate Anopheles stephensi transgenic mosquitoes constitutively secreting human plasminogen activator inhibitor 1 in midgut and/or saliva which leads to inhibition of plasminogen activation and a reduction in oocyst intensity, infection prevalence, and transmission.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal