Jichu yixue yu linchuang (Aug 2022)

Effect of PTOV1 expression on prognosis of colorectal cancer patients

  • XIE Si-an, CHEN Yi-yang, XU Jun-xuan, NING Ting-ting, ZHANG Nan, ZHU Sheng-tao, ZHANG Shu-tian

DOI
https://doi.org/10.16352/j.issn.1001-6325.2022.08.1176
Journal volume & issue
Vol. 42, no. 8
pp. 1176 – 1181

Abstract

Read online

Objective To analyze the effect of over-expression of prostate tumor 1 (PTOV1) on the prognosis of colorectal cancer (CRC) based on bioinformatics, and to demonstrate the effect of PTOV1 on the proliferation of CRC cells. Methods Combined with bioinformatics and biological experiments, PTOV1 expression in CRC tissues and cell lines was analyzed. PTOV1 expression was confirmed by Western blot and RT-qPCR. The effect of PTOV1 on the proliferation of CRC cells was investigated by colony formation assay and EdU assay after transfection by PTOV1 siRNA in SW480 cell line. Results PTOV1 expression was significantly up-regulated in CRC tissues and cell lines (P＜0.05). More biological experiments results showed that PTOV1 as an oncogene was associated with poor prognosis of CRC. In vitro experiments showed that PTOV1 knockdown inhibited the proliferation of SW480 cells(P＜0.05). Conclusions PTOV1 is highly expressed in CRC and is associated with poor prognosis in CRC patients. PTOV1 is a potential prognostic biomarker for the progression of CRC.

Keywords