A protocol for capturing RNA-sensing innate immune receptors in multiple conformations by single-particle cryo-EM

Abstract

Summary: Capturing different conformations of receptor proteins that are complexed with ligands by single-particle cryo-EM facilitates our understanding toward the mechanisms of ligand recognition and receptor activation cascades. Here, we present a protocol for capturing RNA-sensing innate immune receptors, such as RIG-I, in multiple conformations by single-particle cryo-EM. We describe steps for protein-ligand sample preparation, data acquisition, and image processing covering focused three-dimensional classification. This protocol can be adapted to capture the dynamic behavior of other receptors that can be stabilized.For complete details on the use and execution of this protocol, please refer to Wang and Pyle (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Immunology
• Molecular Biology
• Protein Biochemistry
• Protein Expression and Purification
• Structural Biology
• Cryo-EM