CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Mitchell G. Kluesner; Walker S. Lahr; Cara-lin Lonetree; Branden A. Smeester; Xiaohong Qiu; Nicholas J. Slipek; Patricia N. Claudio Vázquez; Samuel P. Pitzen; Emily J. Pomeroy; Madison J. Vignes; Samantha C. Lee; Samuel P. Bingea; Aneesha A. Andrew; Beau R. Webber; Branden S. Moriarity

doi:10.1038/s41467-021-22009-2

Nature Communications (Apr 2021)

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Mitchell G. Kluesner,
Walker S. Lahr,
Cara-lin Lonetree,
Branden A. Smeester,
Xiaohong Qiu,
Nicholas J. Slipek,
Patricia N. Claudio Vázquez,
Samuel P. Pitzen,
Emily J. Pomeroy,
Madison J. Vignes,
Samantha C. Lee,
Samuel P. Bingea,
Aneesha A. Andrew,
Beau R. Webber,
Branden S. Moriarity

Affiliations

Mitchell G. Kluesner: Department of Pediatrics, University of Minnesota
Walker S. Lahr: Department of Pediatrics, University of Minnesota
Cara-lin Lonetree: Department of Pediatrics, University of Minnesota
Branden A. Smeester: Department of Pediatrics, University of Minnesota
Xiaohong Qiu: Department of Pediatrics, University of Minnesota
Nicholas J. Slipek: Department of Pediatrics, University of Minnesota
Patricia N. Claudio Vázquez: Department of Pediatrics, University of Minnesota
Samuel P. Pitzen: Masonic Cancer Center, University of Minnesota
Emily J. Pomeroy: Department of Pediatrics, University of Minnesota
Madison J. Vignes: College of Biological Sciences, University of Minnesota
Samantha C. Lee: Department of Genetics, Cell Biology, and Development, University of Minnesota
Samuel P. Bingea: Department of Pediatrics, University of Minnesota
Aneesha A. Andrew: Department of Genetics, Cell Biology, and Development, University of Minnesota
Beau R. Webber: Department of Pediatrics, University of Minnesota
Branden S. Moriarity: Department of Pediatrics, University of Minnesota

DOI: https://doi.org/10.1038/s41467-021-22009-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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