Zn2+-dependent DNAzymes that cleave all combinations of ribonucleotides

Abstract

Inomata et al. isolate zinc-dependent DNAzymes with compact catalytic core sequences displaying highly versatile hydrolysis activity. The cleavable substrate variation for the DNAzymes (ZincDz2 and ZincDz2-v2) is very broad and the authors further demonstrate their utility by showing ZincDz2 can hydrolyze miR-155 in vitro at three different sites. With their broad substrate tolerance, this study advances the DNAzyme field for RNA cleavage applications.