Potential mechanisms of synthetic endophytic bacterial community to reduce PAHs accumulation in vegetables

Abstract

The functional endophytic bacterial community can effectively degrade polycyclic aromatic hydrocarbons (PAHs), thereby reducing their accumulation in vegetables grown on contaminated sites. However, the biological mechanisms underlying this reduction remain unclear. In this study, we analyzed the efficacy of different colonization methods of the functional endophytic bacterial community m5 in reducing PAHs in vegetables, with a particular focus on the leaf painting method. The results demonstrated that various colonization methods effectively reduced PAHs in vegetables, with leaf painting proving to be a cost-effective and efficient approach. Compared to the non-inoculated control, PAH content in the edible parts of amaranth was reduced by 40.63 % using the leaf painting method. High-throughput sequencing and quantitative PCR revealed that leaf painting altered the bacterial community structure and key components of the bacterial network, enhancing bacterial cooperation. After 20 days of colonization, the abundance of phe and nidA genes in vegetables increased significantly, by tens to hundreds of times, compared to uninoculated controls, thereby promoting the degradation of PAHs in vegetables. This study enhances our understanding of the biological mechanisms by which endophytic bacterial communities reduce PAHs in vegetables.

Keywords

• PAHs
• PAH-degrading genes
• Bacterial community
• Vegetables
• Biodegradation