Food Chemistry Advances (Oct 2023)
The anti-apoptosis effect of isovitexin on human keratinocytes by regulating miR-98-5p/Bcl-2/Bcl-xL and MAPKs/NF-κB signaling pathways
Abstract
Isovitexin is a highly bioactive food-derived flavonoid currently attracting attention for its signal transduction-based antioxidant effects. This study focused on the potential molecular mechanisms by which isovitexin protects HaCaT cells from oxidation-induced apoptosis. It was found that isovitexin significantly reversed oxidative stress-induced apoptosis by reducing ROS level. In addition, isovitexin inhibited apoptosis in a dose-dependent manner by upregulating the expression level of Bcl-xL and Bcl-2 and downregulating the expression level of Bax and caspase-3. It is important to note that the effect of isovitexin may be achieved by targeting miR-98-5p, an upstream target of Bcl-xL and Bcl-2, and overexpression of miR-98-5p counteracted isovitexin-mediated upregulation of antiapoptotic proteins. Isovitexin also inhibited apoptosis by reducing the level of IL-6, IL-1β and TNF-α, which was achieved by regulating MAPKs/NF-κB signaling pathway. Overall, this study confirmed the mechanism of multi-targeted inhibition of apoptosis by targeting miR-98-5p/Bcl-2/Bcl-xL and MAPKs/NF-κB signaling pathway by isovitexin.
