Phase separation-deficient TDP43 remains functional in splicing

Abstract

TDP43 regulates RNA processing and undergoes liquid-liquid phase separation. Here, the authors combine bioinformatic analysis and quantitative measurements of phase properties in living cells to uncover the rules that link the amino acid sequence of TDP43 with its phase properties and find that unexpectedly, mutants of TDP43 that are deficient in phase separation can still function to mediate splicing.