Nature Communications (Jan 2020)
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
- Rory Henderson,
- Maolin Lu,
- Ye Zhou,
- Zekun Mu,
- Robert Parks,
- Qifeng Han,
- Allen L. Hsu,
- Elizabeth Carter,
- Scott C. Blanchard,
- R J Edwards,
- Kevin Wiehe,
- Kevin O. Saunders,
- Mario J. Borgnia,
- Alberto Bartesaghi,
- Walther Mothes,
- Barton F. Haynes,
- Priyamvada Acharya,
- S. Munir Alam
Affiliations
- Rory Henderson
- Department of Medicine, Duke University School of Medicine
- Maolin Lu
- Department of Microbial Pathogenesis, Yale University School of Medicine
- Ye Zhou
- Department of Computer Science, Duke University
- Zekun Mu
- Department of Immunology, Duke University School of Medicine
- Robert Parks
- Duke Human Vaccine Institute, Duke University School of Medicine
- Qifeng Han
- Department of Medicine, Duke University School of Medicine
- Allen L. Hsu
- Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services
- Elizabeth Carter
- Department of Medicine, Duke University School of Medicine
- Scott C. Blanchard
- Department of Physiology and Biophysics, Weill Cornell Medicine
- R J Edwards
- Department of Medicine, Duke University School of Medicine
- Kevin Wiehe
- Department of Medicine, Duke University School of Medicine
- Kevin O. Saunders
- Duke Human Vaccine Institute, Duke University School of Medicine
- Mario J. Borgnia
- Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services
- Alberto Bartesaghi
- Department of Computer Science, Duke University
- Walther Mothes
- Department of Microbial Pathogenesis, Yale University School of Medicine
- Barton F. Haynes
- Department of Medicine, Duke University School of Medicine
- Priyamvada Acharya
- Duke Human Vaccine Institute, Duke University School of Medicine
- S. Munir Alam
- Department of Medicine, Duke University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-019-14196-w
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 14
Abstract
Here, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes. This has important implications for HIV-1 vaccine design.
