Molecular Detection of Clumping factor A gene and Antibiotic Susceptibility Evaluation of Staphylococcus Aureus Isolated from Urinary Tract Infections

Abstract

The present study aimed to isolate and diagnose Staphylococcus Aureus (S. aureus) from clinical specimens of patients infected with urinary tract infections and evaluate the bacteria’s resistance to antimicrobial agents. Additionally, it attempted to study the existence of the clumping factor A (clfA) gene. This study took place in Najaf province, Iraq, from December 2020 to April 2021 and included 40 clinical specimens taken from urine. In order to make an initial diagnosis of S. aureus isolates, microscopic evaluation was used in conjunction with culture and biochemical features. The automatic final diagnostic provided by the VITEK-2 compact system (bioMérieux, France) was utilized, which had a significant advantage. The results showed that 27 (67.5%) isolates gave positive results for S. aureus, 2 (5%) isolates for Streptococcus pyogenes, 6 (15%) isolates for Lactobacillus, and 5 (12.5%) for Escherichia coli. Antibiotic sensitivity test was conducted by disk diffusion methods, in which the isolates showed high resistance to ceftriaxone, as well as erythromycin, and they showed sensitivity to vancomycin, gentamicin, amikacin, and ciprofloxacin. The findings led to a concluding remark that all of the S. aureus isolates were clfA gene positive.

Keywords

• clumping factor a gene
• staphylococcus aureus
• urinary tract
• vitek