Phylogenetic and Phylogeographic Analysis of DENV-1 Genotype Ⅰ in Asian-Pacific Region

Abstract

Objective: To clarify the evolutionary characteristics and transmission mode of DENV-1 genotype I dengue virus in the Asia-Pacific region. Methods: 200 DENV-1 genotype I dengue viruses in the Asia-Pacific region from 2010 to 2014 and 2015 to 2019 were used for phylogenetic analysis and bayesian phylogeographic analysis using a series of molecular biology software. Results: DENV-1 genotype I dengue virus spread frequently between coastal cities in China and Southeast Asian countries. The evolution rate of DENV-1 genotype I dengue virus during 2010-2014 was 5.73×104 (/site/year,95%CI) , and the time to the most recent common ancestor was 1970-09-14.And the evolution rate of virus during 2015-2019 was 5.25×104 (/site/year,95%CI) , and the time to the most recent common ancestor was 1978-10-30.The virus spatial diffusion pathways shows the phenomenon that the dengue virus of this genotype is widespread in the Asia-Pacific region, the spread of the dengue virus between the coastal provinces and some inland provinces of China, and Southeast Asian countries. The study results indicated that the monitoring and preventive measures should be strengthened to avoid the threat of personal safety caused by large-scale spread of DENV-1 in China.