Stem Cell Research (Apr 2024)

Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells

  • Mikaël Boullé,
  • Alix Boucharlat,
  • Ambre Leleu,
  • Céline Banal,
  • Aurélie Coussement,
  • Marcel Hollenstein,
  • Frank Yates,
  • Nathalie Lefort,
  • Fabrice Agou

Journal volume & issue
Vol. 76
p. 103350

Abstract

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Human induced Pluripotent Stem Cells (hiPSCs) represent an invaluable source of primary cells to investigate development, establish cell and disease models, provide material for regenerative medicine and allow more physiological high-content screenings. Here, we generated three healthy hiPSC control lines - IPi001-A/B/C - from primary amniotic fluid cells (AFCs), an infrequently used source of cells, which can be readily obtained from amniocentesis for the prenatal diagnosis of numerous genetic disorders. These AFCs were reprogrammed by non-integrative viral transduction. The resulting hiPSCs displayed normal karyotype and expressed classic pluripotency hallmarks.