<i>Laetiporus sulphureus</i> Mushroom Enhances Cytotoxic Effect of <i>Bifidobacterium animalis</i> spp. <i>lactis</i> on HCT-116 Cells in a Co-Culture System

Abstract

The study aimed to test the effect of probiotic Bifidobacterium animalis spp. lactis (BAL) on the HCT-116 cell line viability and to compare its effect with co-treatment BAL/Laetiporus sulphureus (EALS). The trypan blue staining method was used to estimate HCT-116 viability. The levels of NO2− were determined using 0.1% N-(1-naphthyl) ethylenediamine, as well as 1% sulfanilic acid. The determination of H2O2 was based on the oxidation of phenol red. Our results showed the significant cytotoxicity of the BAL on the HCT-116 cells in a co-culture system, while the BAL/EALS co-treatment further enhanced the cytotoxicity on the HCT-116 cells. We detected higher H2O2 and NO2− values in treatments with BAL, especially in the BAL/EALS co-treatment. The death of the HCT-116 cells may be due to elevated levels of H2O2 and NO2− and their products (peroxynitrites).

Keywords

• edible mushroom
• probiotics
• <i>Bifidobacterium</i>
• cancer cells
• extract
• H₂O₂