Influence of spirocarbone and derivates of pyrrolopyrimidinedions on phisical and chemical properties of the hemoglobin ligand forms of blood in vitro

Abstract

The effect of heterocyclic substance spirocarbon and pyrrolopyrimidinedione derivatives was studied on the hemoglobin ligand forms dynamics (RHb, HbO2, HbCO, SHb, MetHb), its basic stability, methemoglobin peroxidase activity and spectrophotometric characteristics of CNMetHb-Coomassi G-250 in the healthy donors’ blood. It was shown that spirocarbon causes a slight HbO2 decrease. In the experiments with pyrrolopyrimidindion derivatives the everage HbO2 values are in the range of control. There was carried out an analysis of HbO2, MetHb, CNMetHb electron spectra and the electron spectra of the complexes CNMetHb-Coomassi G-250. It was testified that spirocarbon causes a certain increase of basic stable hemoglobin and decrease of peroxydase activity. The pyrrolopyrimidinedione derivatives result in increasing of methemoglobin peroxydase activity in the tested samples.

Keywords

• hemoglobin
• ligand forms
• electron spetra
• spirocarbon
• pyrrolopyri­mi­dine­dions derivatives
• basic stability
• peroxydase