Ciência Rural ()

Physicochemical characterization and evaluation of in vitro and in vivo toxicity of goldenberry extract nanoemulsion

  • Suelen Santos da Silva,
  • Maiara Taís Bazana,
  • Cassandra de Deus,
  • Marina Lopes Machado,
  • Larissa Marafiga Cordeiro,
  • Félix Alexandre Antunes Soares,
  • Daniele Rubert Nogueira Libreloto,
  • Clarice Madalena Bueno Rolim,
  • Cristiano Ragagnin de Menezes,
  • Cristiane Franco Codevilla

DOI
https://doi.org/10.1590/0103-8478cr20190015
Journal volume & issue
Vol. 49, no. 8

Abstract

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ABSTRACT: Oil-in-water (O/W) nanoemulsion containing goldenberry extract was elaborated using a high-energy ultrasonic bath method. Physicochemical characterization of the formulation was carried out by determining pH, mean droplet diameter, polydispersity index (PDI) and zeta potential. Nanoemulsion toxicity was assessed using in vitro assays with tumor and non-tumor cell lines, and in vivo using Caenorhabditis elegans. The pH of the nanoemulsion was 3.84, the mean droplet diameter was 268 ± 7 nm, PDI 0.113 and zeta potential -13.94 mV. Results of the cytotoxicity assays employing non-tumor cells indicated that the extract associated or not with nanoemulsion maintained cell viability at different concentrations tested. In the assays using tumor lineage, it is observed that the nanoemulsion containing the extract had higher antitumor activity than the free extract. As for the in vivo tests, there was no change in the survival rate of the worms.

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