Polymorphisms of Genes Involved in Endothelial Dysfunction in the Yakuts with COPD and Metabolic Syndrome

Abstract

The aim of our research was to study the association of the -675 4G/5G (rs1799889) SNP of the SERPINE1 (PAI-1) gene and the Arg353Gln G>A (rs6046) SNP of the F7 gene with metabolic syndrome (MetS) in the Yakuts with COPD. Methods and Results: A molecular-genetic examination was conducted in 200 COPD patients of Yakut ethnicity. The main group (MG) consisted of 100 COPD patients with MetS, the comparison group (CG) included 100 COPD patients without MetS. The distribution of genotypes of studied SNPs was in Hardy-Weinberg equilibrium in all cases. Studying the SERPINE1 -675 4G/5G SNP, we found the prevalence of a 4G allele in MG, compared to CG (OR=1.84, 95% CI 1.23–2.74; χ2=9.06, P=0.003). Incidence of the homozygous 4G/4G mutation was rather high in MG, compared to CG (OR=2.35, 95%CI 1.24–4.44; χ2=9.31, P=0.002). According to our data, the presence of MetS in Yakut patients with COPD has been associated with the carrier of the 4G/4G genotype. Studying the F7 Arg353Gln SNP, we found the prevalence of an Arg253 allele in both groups (0.72 in MG and 0.71 in CG; χ2=0.01, P=0.91). The homozygous Gln353/Gln353 mutant genotype was rare in both groups (0.12 in MG and 0.10 in CG; OR=1.23, 95%CI 0.50–2.99; χ2=0.01, P=0.92). In our study, the F7 Arg353Gln SNP was not associated with protection against MetS in COPD patients.

Keywords

• SERPINE1 gene
• F7 gene
• metabolic syndrome
• chronic obstructive pulmonary disease