IN VITRO IHBITION OF PSEUDOMONAS FLUORESCENS BACTERIA BY USING CURRY TREE (MURRAYA KOENIGII) CRUDE EXTRACT

Abstract

Pseudomonas fluorescens is a bacterium that frequently infects fish living in freshwater environments. These bacteria can cause diseases that result in mass fish mortality. Besides, antibiotic treatment of the deseases results in antibiotic-resistant bacteria and antibiotic residues. Thus, alternative efforts utilizing natural ingredients such as curry tree (Murraya koenigii) extract were conducted. Curry tree contains active antibacterial compounds. A completely randomized design (CRD) was used in this study, with doses of (A) 125 ppm, (B) 150 ppm, (C) 175 ppm, (D) 200 ppm, and (E) 225 ppm, with a positive control using 30 ppm tetracycline antibiotics, and a negative control without extract. Each treatment was carried out three times. The crude curry tree extract (M. koenigii) inhibited the growth of P. fluorescens bacteria, forming a linear pattern with the equation y= 0.033x + 3.30 and the coefficient value R2= 0.82. Treatment (E) with 250 ppm had the largest clear zone diameter with an average inhibition zone of 11.20±1.05 mm. Meanwhile, treatment (A) with 125 ppm had the smallest clear zone with an average of 7.63±0.98 mm. This study found that administering curry tree extract (M. koenigii) affected the inhibition of P. fluorescens bacteria. In this study, the best dose of curry tree extract was 225 ppm, with an average inhibition zone diameter of 11.20 mm.

Keywords

• curry tree (murraya koenigii)
• pseudomonas fluorescens
• infection
• fish