Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts

Watcharapong Mitsuwan; Chea Sin; Samell Keo; Suthinee Sangkanu; Maria de Lourdes Pereira; Tajudeen O. Jimoh; Cristina C. Salibay; Muhammad Nawaz; Roghayeh Norouzi; Abolghasem Siyadatpanah; Christophe Wiart; Polrat Wilairatana; Polydor Ngoy Mutombo; Veeranoot Nissapatorn

Heliyon (May 2021)

Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts

Watcharapong Mitsuwan,
Chea Sin,
Samell Keo,
Suthinee Sangkanu,
Maria de Lourdes Pereira,
Tajudeen O. Jimoh,
Cristina C. Salibay,
Muhammad Nawaz,
Roghayeh Norouzi,
Abolghasem Siyadatpanah,
Christophe Wiart,
Polrat Wilairatana,
Polydor Ngoy Mutombo,
Veeranoot Nissapatorn

Affiliations

Watcharapong Mitsuwan: School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand; Akkhraratchakumari Veterinary College and Research Center of Excellence in Innovation of Essential Oil, Walailak University, Nakhon Si Thammarat, Thailand
Chea Sin: Faculty of Health Sciences, University of Puthisastra, Phnom Penh, Cambodia
Samell Keo: Academic Center for Education and Training (ACET), Phnom Penh, Cambodia
Suthinee Sangkanu: School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand
Maria de Lourdes Pereira: CICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro, Aveiro, Portugal
Tajudeen O. Jimoh: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Department of Biochemistry, Habib Medical School, Islamic University in Uganda, Kampala, Uganda
Cristina C. Salibay: College of Science and Computer Studies, De La Salle University-Dasmarinas, Dasmarinas City, Cavite, Philippines
Muhammad Nawaz: Department of Nano-Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
Roghayeh Norouzi: Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Abolghasem Siyadatpanah: Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran
Christophe Wiart: School of Pharmacy, University of Nottingham Malaysia Campus, Selangor, Malaysia
Polrat Wilairatana: Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Thailand
Polydor Ngoy Mutombo: Independent Consultant, Neglected Tropical Diseases, Melbourne, Victoria, Australia
Veeranoot Nissapatorn: School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand; Corresponding author.

Journal volume & issue: Vol. 7, no. 5
p. e06976

Abstract

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Plants with medicinal properties have been used in the treatment of several infectious diseases, including Acanthamoeba infections. The medicinal properties of Cambodian plant extracts; Annona muricata and Combretum trifoliatum were investigated against Acanthamoeba triangularis. A total of 39 plant extracts were evaluated and, as a result, 22 extracts showed positive anti-Acanthamoeba activity. Of the 22 extracts, 9 and 4 extracts showed anti-Acanthamoeba activity against trophozoites and cysts of A. triangularis, respectively. The minimum inhibitory concentration of A. muricata and C. trifoliatum extracts against trophozoites and cysts was 500 and 1,000 μg/mL, respectively. The combination of A. muricata at 1/4×MIC with chlorhexidine at 1/8×MIC demonstrated a synergistic effect against trophozoites, but partial synergy against cysts. A 40% reduction in trophozoites and 60% of cysts adhered to the plastic surface treated with both extracts at 1/2×MIC were noted comparing to the control (P < 0.05). Furthermore, a reduction of 80% and 90% of trophozoites adhered to the surface was observed after pre-treatment with A. muricata and C. trifoliatum extracts, respectively. A 90% of cysts adhered to the surface was decreased with pre-treatment of A. muricata at 1/2×MIC (P < 0.05). A 75% of trophozoites and cysts from Acanthamoeba adhered to the surface were removed after treatment with both extracts at 4×MIC (P < 0.05). In the model of contact lens, 1 log cells/mL of trophozoites and cysts was significantly decreased post-treatment with both extracts compared to the control. Trophozoites showed strong loss of acanthopodia and thorn-like projection pseudopodia, while cysts demonstrated retraction and folded appearance treated with both extracts when observed by SEM, which suggests the potential benefits of the medicinal plants A. muricata and C. trifoliatum as an option treatment against Acanthamoeba infections.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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