Zhongguo linchuang yanjiu (Feb 2024)

lncRNA ANCR regulating the effects of miR-497-5p on vascular lumen formation and apoptosis of gastric cancer cells

  • ZHU Tingting, XIA Yihan, SUN Shasha, GAO Peng, QIU Wensheng

DOI
https://doi.org/10.13429/j.cnki.cjcr.2024.02.011
Journal volume & issue
Vol. 37, no. 2
pp. 216 – 222

Abstract

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Objective To explore the effects of lncRNA ANCR regulating miR-497-5p on vascular lumen formation, apoptosis and PI3K/AKT signaling pathway of gastric cancer cells. Methods Gastric cancer cells were collected and divided into gastric cancer cell (GC) group, gastric cancer cell +lncRNA ANCR-NC (AN) group, gastric cancer cell + lncRNA ANCR overexpressed plasmid (AM) group, gastric cancer cell + lncRNA ANCR siRNA (AI) group, gastric cancer cells + miR-497-5p-NC (MN) group, gastric cancer cells + miR-497-5p siRNA (MI) group, gastric cancer cells +miR-497-5p mimics (MM) group, and gastric cancer cells + lncRNA ANCR siRNA+miR-497-5p mimics (IM) group. Vascular lumen formation was observed by lumen formation assay, cell apoptosis was detected by PI staining method, and protein expression of PI3K and AKT was detected by western blotting. The interaction between lncRNA ANCR and miR-497-5p was confirmed by double luciferase reporting assay. Results Compared with AN group and MN group, the apoptosis rate of AM group and MI group was significantly decreased (P＜0.05), the number of vascular lumen formation and the expression of PI3K and AKT protein were significantly increased (P＜0.05). Compared with AM and MI groups, the apoptosis rate of AI and MM groups was significantly increased (P＜0.05), while the number of vascular lumen formation and the expression of PI3K and AKT protein were significantly decreased (P＜0.05). Compared with AI and MM groups, the apoptosis rate of IM group was significantly increased (P＜0.05), the number of vascular lumen formation and the expression of PI3K and AKT protein were significantly decreased (P＜0.05). The results of double luciferase report showed that transfection of lncRNA ANCR could significantly reduce the luciferase activity of miR-497-5p-3′-UTR-WT (P＜0.05), but had no significant effect on mutant genes (P＞0.05). Conclusion Inhibition of lncRNA ANCR can effectively inhibit the formation of vascular lumen, promote cell apoptosis, and inhibit the expression of PI3K/AKT signaling pathway in gastric cancer cells, which may be related to the targeted activation of miR-497-5p.

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