Evaluation of invitro α-amylase and α-glucosidase inhibitory potential of N2O2 schiff base Zn complex

Abstract

The evaluation of the antioxidant, antibacterial and enzyme inhibition effects of N2O2 Schiff base Zn complex was investigated. The percentage scavenging activity of hydroxyl radical (IC50-0.21 μg), ABTS (IC50-0.19 μg) and DPPH (IC50-0.25 μg) shows that ZnL complex had a potential antioxidant activity. The antibacterial activity of ZnL complex was evaluated. Results showed that MIC value against Staphylococcus aureus and Salmonella typhi was 100 and 200 μg/ml, respectively. ZnL complex showed strong inhibition toward α-amylase and α-glucosidase with an IC50 value of 0.18 and 0.23 μg, respectively. The inhibition mechanism was analyzed with LB and Dixon plots. The ZnL complex could be a mixed noncompetitive and noncompetitive inhibitor with Ki values of 77.8 and 31.6 μg for α-amylase and α-glucosidase, respectively. The preliminary assessment of ZnL complex showed that they can be used as an antioxidant, antibacterial and antidiabetic agents.

Keywords

• ZnL complex
• Antioxidant
• Antibacterial
• Kinetic studies