Using Bio-Functionalized Magnetic Nanoparticles and  Dynamic Nuclear Magnetic Resonance to Characterize the Time-Dependent Spin-Spin Relaxation Time for Sensitive  Bio-Detection

Shu-Hsien Liao; Kuen-Lin Chen; Chun-Ming Wang; Jen-Jie Chieh; Herng-Er Horng; Li-Min Wang; C. H. Wu; Hong-Chang Yang

doi:10.3390/s141121409

Sensors (Nov 2014)

Using Bio-Functionalized Magnetic Nanoparticles and Dynamic Nuclear Magnetic Resonance to Characterize the Time-Dependent Spin-Spin Relaxation Time for Sensitive Bio-Detection

Shu-Hsien Liao,
Kuen-Lin Chen,
Chun-Ming Wang,
Jen-Jie Chieh,
Herng-Er Horng,
Li-Min Wang,
C. H. Wu,
Hong-Chang Yang

Affiliations

Shu-Hsien Liao: Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Kuen-Lin Chen: Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan
Chun-Ming Wang: Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Jen-Jie Chieh: Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Herng-Er Horng: Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
Li-Min Wang: Graduate Institute of Applied Physics and Department of Physics, National Taiwan University, Taipei 106, Taiwan
C. H. Wu: Department of Physics, National Chung Hsing University, Taichung 402, Taiwan
Hong-Chang Yang: Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan

DOI: https://doi.org/10.3390/s141121409
Journal volume & issue: Vol. 14, no. 11
pp. 21409 – 21417

Abstract

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In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe3O4 particles labeled as Fe3O4-antiCRP. It was found the time-dependent spin-spin relaxation time, T2, of protons decreases as time evolves. Additionally, the ΔT2 of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 μg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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