Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD)

Abstract

A 32-year old Obsessive-Compulsive Disorder (OCD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in OCD.Resource Table.Unlabelled TableUnique stem cell line identifierTUSMi004-AAlternative name(s) of stem cell lineXWInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, [email protected] of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell SourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic ModificationNOType of ModificationN/AAssociated diseaseObsessive-Compulsive Disorder(OCD)Gene/locus5-HT1A/chr 5:63258565, rs6295 C-G,LHPP/chr 10:126244970, rs35936514 C-T,HTR1A/chr 63,255,991, rs878567 C-TMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi004-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG)0.016.01.1).