High-density cultivation of Phaffia rhodozyma SFAS-TZ08 in sweet potato juice for astaxanthin production

Abstract

Background: Astaxanthin is an important commercially valuable secondary metabolite produced during fermentation of the yeast Phaffia rhodozyma that can be used in aquatic feedstock. Results: In this study, we used sweet potato juice (SPJ), the by-product of sweet potato starch, to obtain a high-density culture of P. rhodozyma. We confirmed that hydrochloric acid deproteinized SPJ is suitable for the culture of P. rhodozyma, and using this as a substrate, supplemented with 0.05% yeast extract, we performed batch fermentation in a 5 L fermenter. Compared to shaking flask fermentation, we obtained an 18.86% increase in yeast biomass and a 32.5% increase in astaxanthin yield using the batch process. After culturing P. rhodozyma in a 5 L fermenter for 120 h, we achieved biomass and astaxanthin yield of 45.2 g/L and 19.465 mg/L, respectively. Conclusions: In this study, we found that deproteinized SPJ was the most suitable for P. rhodozyma through experiments on different cultivars and different processing stages of SPJ. On the basis of deproteinized SPJ, supplemented with yeast extract, the biomass and astaxanthin yield reached a high level. The optimized system can substantially reduce the costs of raw material for astaxanthin yield by P. rhodozyma and enhance the comprehensive utilization of sweet potato resources.How to cite: Zhang C, Zhao X, Yao M, et al. High-density cultivation of Phaffia rhodozyma SFAS-TZ08 in sweet potato juice for astaxanthin production. Electron J Biotechnol 2022;61. https://doi.org/10.1016/j.ejbt.2022.09.007.

Keywords

• Antioxidant
• Aquatic feedstock
• Astaxanthin
• Astaxanthin production
• Fed-batch fermentation
• Natural carotenoids