Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Abstract

A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and develop a model which ranks off-target sites.