Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC

Abstract

CRISPR-Cas9 has been utilized, through the fusion of catalytic dead nuclease with chromatin-remodellers, to modify the epigenetic state of specific loci. Here the authors manipulate locus-specific histone acetylation and describe the parameters that need to be considered for its use.