Evaluation of mice infected to Salmonella Spp in Poultry farms of Tehran Province

Abstract

In this survey, 290 mice and rats fecal samples from commercial layer and broiler poultry houses were tested for Salmonella sp. presence. All samples were cultured on Selenite F broth media and passaged on SS agar and McConkey agar. The suspected colonies were cultured on Urea and TSI agars to be confirmed as Salmonella sp.. Finally, Salmonella isolates were identified genetically and biochemically by PCR and conventional methods, respectively. Serogrouping and Antibiotic resistance profiling were done for further differentiation of isolates. Twenty eight (9.65%) Salmonellas were isolated from (out of) 290 samples. Eight (28.6%), seven (25%), four (14.3%), and two (7.2%) isolates were located in serogroups C, D, B and E, respectively. Seven isolates (25%) belonged to Arizona subspecies and just one non-motile serogroup D Salmonella was isolated. All isolates were sensitive to enrofloxacin, difloxacin, norfloxacin, chloramphenicol and florfenicol, but they were resistant to sulfamethoxazole, trimethoprim and neomycin in decreasing order. In addition to former surveys, this study confirmed the role of mice and rats in spreading of Salmonella spp. in poultry farms. In conclusion it is essential to take appropriate measurements (measures) for pest management in poultry houses to approach the prevention of some bacterial infection like (such as ) salmonellosis.

Keywords

• Salmonella sp
• Mice
• Poultry Farm