Jichu yixue yu linchuang (Aug 2021)
Wnt-5a/JNK is involved in PM2.5-induced expression of IL-6 and TNF-α of human bronchial epithelial cell line 16HBE
Abstract
Objective To explore whether PM2.5 induces the secretion of IL-6 and TNF-α in 16HBE via the Wnt-5a/JNK signaling pathway. Methods The research was implemented by rats and 16HBE cells. Rats were divided into control group and motor vehicle exhaust(MVE) group(concentration 1.5 mg/m2,2 h/day); 16HBE cells were divided into control group,PM2.5(20 μg/mL)group,control siRNA+PM2.5 group,Wnt-5a siRNA+PM2.5 group,Wnt-5a siRNA+control group,JNK inhibitor SP600125+PM2.5 group and SP600125+control group. The expressions of p-JNK and p-c-Jun in lung tissues were examined by immune-histochemistry method. The expressions of Wnt-5a,JNK,c-Jun,p-JNK and p-c-Jun were measured by Western blot. The secretions of IL-6 and TNF-α were assessed by ELISA. Results Compared with the control rat group, MVE group exhibited significantly up-regulated the expression of p-JNK and p-c-Jun(P＜0.05); Compared with the 16HBE cell control group,PM2.5 group significantly up-regulated the expressions of Wnt-5a,p-JNK and p-c-Jun and the secretions of IL-6 and TNF-α(P＜0.05);Compared with the PM2.5 group, Wnt-5a siRNA+PM2.5 group blocked these effects(P＜0.05). Furthermore,SP600125+PM2.5 group showed a significantly decreased expression of p-c-Jun and the secretion of IL-6 and TNF-α by 16HBE cells(P＜0.05). Conclusions PM2.5 induces the secretions of IL-6 and TNF-α by 16HBE cells via Wnt-5a/JNK signaling pathway.
