Super-resolution microscopy based on parallel detection

Zhimin Zhang; Shaocong Liu; Liang Xu; Yubing Han; Cuifang Kuang; Yong Liu; Xiang Hao; Hongqin Yang; Xu Liu

doi:10.1142/S1793545819500238

Journal of Innovative Optical Health Sciences (Nov 2019)

Super-resolution microscopy based on parallel detection

Zhimin Zhang,
Shaocong Liu,
Liang Xu,
Yubing Han,
Cuifang Kuang,
Yong Liu,
Xiang Hao,
Hongqin Yang,
Xu Liu

Affiliations

Zhimin Zhang: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Shaocong Liu: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Liang Xu: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Yubing Han: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Cuifang Kuang: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Yong Liu: College of Electronics and Information Engineering, Shanghai University of Electric Power, Shanghai 200090, P. R. China
Xiang Hao: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Hongqin Yang: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China
Xu Liu: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China

DOI: https://doi.org/10.1142/S1793545819500238
Journal volume & issue: Vol. 12, no. 6
pp. 1950023-1 – 1950023-7

Abstract

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Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using pixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.

Published in Journal of Innovative Optical Health Sciences

ISSN: 1793-5458 (Print); 1793-7205 (Online)
Publisher: World Scientific Publishing
Country of publisher: Singapore
LCC subjects: Technology; Science: Physics: Optics. Light
Website: http://www.worldscientific.com/worldscinet/jiohs

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