Antioxidant and free radicals species in the aqueous humor of patients with age-related cataract

Brunet Stefan; Babić Nikola; Davidović Sofija; Miljković Aleksandar; Knezi Nikola; Čanadanović Vladimir

doi:10.2298/SARH230619092B

Srpski Arhiv za Celokupno Lekarstvo (Jan 2023)

Antioxidant and free radicals species in the aqueous humor of patients with age-related cataract

Brunet Stefan,
Babić Nikola,
Davidović Sofija,
Miljković Aleksandar,
Knezi Nikola,
Čanadanović Vladimir

Affiliations

Brunet Stefan: University Clinical Center of Vojvodina, Eye Clinic, Novi Sad, Serbia + University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Babić Nikola: ORCiD; University Clinical Center of Vojvodina, Eye Clinic, Novi Sad, Serbia + University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Davidović Sofija: ORCiD; University Clinical Center of Vojvodina, Eye Clinic, Novi Sad, Serbia + University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Miljković Aleksandar: ORCiD; University Clinical Center of Vojvodina, Eye Clinic, Novi Sad, Serbia + University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Knezi Nikola: ORCiD; University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
Čanadanović Vladimir: ORCiD; University Clinical Center of Vojvodina, Eye Clinic, Novi Sad, Serbia + University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia + Academy of Medical Science of the Serbian Medical Society, Beograd, Serbia

DOI: https://doi.org/10.2298/SARH230619092B
Journal volume & issue: Vol. 151, no. 11-12
pp. 676 – 683

Abstract

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Introduction/Objective. Age-related cataract is a significant cause of visual impairment worldwide. Oxidative damage and the effects of free radical species are considered important in the etiopathogenesis of cataracts. The aim of this study was to evaluate the antioxidative capacity and oxidative stress in the aqueous humor (AH) according to age and cataracts maturity. Methods. Clinical and biochemical researches were carried out in 55 patients with age-related cataract. According to the cataract maturity, patients were classified into incipient (cortical – group C, 18 patients; nuclear – group N, 20 patients; mature – group M, 17 patients). In order to evaluate the impact of age patients within each group were divided into Group I (65–69 years) and Group II (70 ≥ years). The antioxidant activity of AH was measured by the reduction power method and the activity of glutathione peroxidase (GPx) spectrophotometrically. Changes in the concentrations of hydroxyl and ascorbyl radicals were detected by electron spin resonance spectroscopy. Results. Both reduction power and GPx activity were significantly (p < 0.001) reduced in group N compared to group C and in group M compared to group N. Concentrations of hydroxyl (29.45 ± 1.01% in group C, 38.12 ± 1.29% in group N, and 74.14 ± 2.52% in group M) and ascorbyl radicals (26.12 ± 0.89% in group C, 41.15 ± 1.39% in group N, and 83.56 ± 2.84% in group M) increased significantly (p < 0.05) with progression of age-related cataract. Significant negative correlation (r = -0.759, p < 0.05) was determined between concentrations of HO• and content of GPx. Conclusion. Our research proved that the level of oxidative stress in the AH is significantly affected during aging and cataract progression, The obtained data support the hypothesis that during aging, depending on the maturity of the cataract, the antioxidant capacity in the AH decreases due to an increase in the concentration of reactive HO•.

Published in Srpski Arhiv za Celokupno Lekarstvo

ISSN: 0370-8179 (Print); 2406-0895 (Online)
Publisher: Serbian Medical Society
Country of publisher: Serbia
LCC subjects: Medicine
Website: http://www.srpskiarhiv.rs/

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