Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae

Jiao Feng; Zhe Yin; Qiangyuan Zhao; Yachao Zhao; Defu Zhang; Xiaoyuan Jiang; Weili Wu; Weijun Chen; Hui Wang; Yajun Song; Yigang Tong; Jinglin Wang; Yanjun Li; Dongsheng Zhou

doi:10.1038/s41598-017-06283-z

Scientific Reports (Jul 2017)

Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae

Jiao Feng,
Zhe Yin,
Qiangyuan Zhao,
Yachao Zhao,
Defu Zhang,
Xiaoyuan Jiang,
Weili Wu,
Weijun Chen,
Hui Wang,
Yajun Song,
Yigang Tong,
Jinglin Wang,
Yanjun Li,
Dongsheng Zhou

Affiliations

Jiao Feng: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Zhe Yin: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Qiangyuan Zhao: Laboratory Department, Navy General Hospital
Yachao Zhao: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Defu Zhang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Xiaoyuan Jiang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Weili Wu: Beijing Institute of Genomics, Chinese Academy of Sciences
Weijun Chen: Beijing Institute of Genomics, Chinese Academy of Sciences
Hui Wang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Yajun Song: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Yigang Tong: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Jinglin Wang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Yanjun Li: Laboratory Department, Navy General Hospital
Dongsheng Zhou: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology

DOI: https://doi.org/10.1038/s41598-017-06283-z
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

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Abstract This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones of p0716-KPC/p12181-KPC contained two different replicons (belonging to a novel IncFII subtype and the Rep_3 family), the IncFIIK and IncFIIY maintenance regions, and conjugal transfer gene sets from IncFIIK-type plasmids and unknown origins. p0716-KPC and p12181-KPC carried similar three accessory resistance regions, namely ΔTn6209, a MDR region, and the bla KPC-2 region. Resistance genes bla KPC-2, mph(A), strAB, aacC2, qacEΔ1, sul1, sul2, and dfrA25, which are associated with transposons, integrons, and insertion sequence-based mobile units, were located in these accessory regions. p0716-KPC carried two additional resistance genes: aphA1a and bla TEM-1. Together, our analyses showed that p0716-KPC and p12181-KPC belong to a novel IncFII subtype and display a complex chimeric nature, and that the carbapenem resistance gene bla KPC-2 coexists with a lot of additional resistance genes on these two plasmids.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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