Frontiers in Plant Science (Sep 2022)
Fine mapping of the Hairy glume (Hg) gene in a chromosome variation region at the distal terminus of 1AS
- Wei Luo,
- Wei Luo,
- Wei Luo,
- Jieguang Zhou,
- Jieguang Zhou,
- Jiajun Liu,
- Jiajun Liu,
- Yanlin Liu,
- Yang Mu,
- Huaping Tang,
- Qiang Xu,
- Qiang Xu,
- Mei Deng,
- Qiantao Jiang,
- Qiantao Jiang,
- Guoyue Chen,
- Guoyue Chen,
- Pengfei Qi,
- Pengfei Qi,
- Jirui Wang,
- Jirui Wang,
- Yunfeng Jiang,
- Yunfeng Jiang,
- Zhongxu Chen,
- Zhi Zheng,
- Yuming Wei,
- Yuming Wei,
- Youliang Zheng,
- Xiujin Lan,
- Xiujin Lan,
- Jian Ma,
- Jian Ma
Affiliations
- Wei Luo
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Wei Luo
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Wei Luo
- College of Life Science and Technology, Xinjiang University, Ürümqi, Xinjiang, China
- Jieguang Zhou
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Jieguang Zhou
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jiajun Liu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Jiajun Liu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yanlin Liu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yang Mu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Huaping Tang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Qiang Xu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Qiang Xu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Mei Deng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Qiantao Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Qiantao Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Guoyue Chen
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Guoyue Chen
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Pengfei Qi
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Pengfei Qi
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jirui Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Jirui Wang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yunfeng Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Yunfeng Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Zhongxu Chen
- Department of Life Science, Chengdu Tcuni Technology, Chengdu, Sichuan, China
- Zhi Zheng
- Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, St. Lucia, QLD, Australia
- Yuming Wei
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Yuming Wei
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Youliang Zheng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Xiujin Lan
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Xiujin Lan
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jian Ma
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China
- Jian Ma
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- DOI
- https://doi.org/10.3389/fpls.2022.1006510
- Journal volume & issue
-
Vol. 13
Abstract
Trichomes are differentiated epidermal cells and exist on above-ground organs of nearly all land plants with important roles in resistance to a wide range of biotic and abiotic stresses. We attempted to obtain candidate gene (s) for Hairy glume (Hg), responsible for the trichome on wheat glume, by using bulked segregant exome capture sequencing (BSE-Seq), while Hg was only mapped in 0.52–3.26 Mb of 1AS. To further fine map this gene and identify candidate genes in this region, a near isogenic line-derived population consisting of 2,050 F2 lines was generated in the present study. By analyzing this population, Hg was fine mapped into a 0.90 cM region covering a physical distance of ~825.03 Kb encompassing 6 high- and 23 low-confidence genes in the reference genome of Chinese Spring. A presence-absence variation was identified in the fine mapping region through analyses of sequence-tagged sites markers and genome sequences of the hairy glume parent of the near isogenic lines. The results presented here will be useful for further cloning Hg in wheat.
Keywords
