Development of voltammetric melanin sensor with 2,5-dimethylfuran modified platinum electrode

Abstract

In this study, an electrochemical sensor was developed for the electrochemical determination of melanin. For this purpose, phosphate buffer salt (PBS, pH 7.00) solution was used as an electrolyte, 2,5-dimethylfuran (2,5-DMF) as a monomer, Pt wire as the auxilary electrode, Ag/AgCl electrode as the reference electrode and a platinum electrode as the working electrode. The modification of the electrode was performed by polymerization of 2,5-DMF at the bare Pt electrode surface using the cyclic voltammetry method. Parameters such as film thickness, scan rate and pulse amplitude with prepared 2,5-DMF modified platinum electrode (p(2,5-DMF)/PtE) were optimized by square wave voltammetry (SWV) in PBS buffer containing melanin. In optimum conditions, the anodic peak height showed a linear increase with the melanin concentration. The limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 0.088 mmol/l and 0.292 mmol/l, respectively, with melanin concentrations ranging from 0.10 to 2.20 mmol/l and the correlation coefficient (R2) was calculated as 0.9985. Stability of the prepared sensor was found to be 99.63% by using the reproducibility experiments (n = 15).

Keywords

• square wave voltammetry
• melanin
• sensor
• 2
• 5-dimethylfuran