Fermentation broth of Serpula similis protect cells from oxidative stress-induced senescence via AMPK-dependent autophagy restoration

Abstract

Serpula similis is an interesting member of Hymenomycetes in association with bamboo clumps. However, the functions of Serpula similis on cellular senescence are not clear yet. In this study, the protective effect of fermentation broth of Serpula similis (FeBrSS) on cellular senescence-induced by oxidative stress and the potential mechanism was studied. The stress-induced premature senescence model was established by hydrogen peroxide, and model cells were subjected to the intervention with FeBrSS. As a result, we found that FeBrSS administration certainly repressed activity of senescence associated β-Galactosidase and expression of senescence-associated genes. Interestingly, the effects of FeBrSS are related to the restoration of impaired autophagy via activation of AMP-activated protein kinase (AMPK) other than via mTORC1 pathway. Together, our study demonstrated for the first time that FeBrSS have the effects of inhibiting senescence via autophagy restoration which occurs due to activation of AMPK pathway, and therefore, supported that autophagy plays a critical role in anti-aging. Our findings expanded the list of fermentation broth of fungus of significance and may provide new evidence or strategies for developing new drugs for anti-aging.

Keywords

• AMPK
• autophagy
• fermentation broth of Serpula similis
• oxidative stress
• senescence