BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL

Abstract

β-Tricalcium Phosphate (β–TCP) have been widely used as an implant materials. It has been successfully produced locally using two different method which is hydrothermal and precipitation. The aim of the study was to determine the biocompatibility of β-TCP prepared by hydrothermal and precipitation method with normal human osteoblast (NHOst) cells. For this purpose cytotoxicity of the material was assessed using an Alamar Blue method to determine the viability of NHOst cells grown with extracts of β-TCP in various concentrations. In addition NHOst grown on β-TCP ceramics were examined under an inverted microscope after 4, 24, 48 and 72 hours to verify cell attachment. Staining was done using Calcein AM and Ethidium homodimers to assess viability using a Confocal Laser Scanning Microscope (CLSM). The results showed that neither hydrothermal β-TCP nor precipitation β-TCP were cytotoxic with either of the method applied.

Keywords

• β-Tricalcium phosphate
• Cytotoxicity
• Attachment
• CLSM
• Osteoblast