Rapid and deep-scale ubiquitylation profiling for biology and translational research

Namrata D. Udeshi; Deepak C. Mani; Shankha Satpathy; Shaunt Fereshetian; Jessica A. Gasser; Tanya Svinkina; Meagan E. Olive; Benjamin L. Ebert; Philipp Mertins; Steven A. Carr

doi:10.1038/s41467-019-14175-1

Nature Communications (Jan 2020)

Rapid and deep-scale ubiquitylation profiling for biology and translational research

Namrata D. Udeshi,
Deepak C. Mani,
Shankha Satpathy,
Shaunt Fereshetian,
Jessica A. Gasser,
Tanya Svinkina,
Meagan E. Olive,
Benjamin L. Ebert,
Philipp Mertins,
Steven A. Carr

Affiliations

Namrata D. Udeshi: Broad Institute of MIT and Harvard
Deepak C. Mani: Broad Institute of MIT and Harvard
Shankha Satpathy: Broad Institute of MIT and Harvard
Shaunt Fereshetian: Broad Institute of MIT and Harvard
Jessica A. Gasser: Broad Institute of MIT and Harvard
Tanya Svinkina: Broad Institute of MIT and Harvard
Meagan E. Olive: Broad Institute of MIT and Harvard
Benjamin L. Ebert: Broad Institute of MIT and Harvard
Philipp Mertins: Broad Institute of MIT and Harvard
Steven A. Carr: Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-019-14175-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

Read online

Comprehensive protein ubiquitylation profiling by mass spectrometry typically requires large sample amounts, limiting its applicability to tissue samples. Here, the authors present an optimized proteomics method that enables multiplexed ubiquitylome analysis of cells and tumor tissue samples.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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